In Situ Hybridization

In Situ Hybridization

A Practical Approach

eBook - 1998
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Oxford University Press
In situ hybridization is used to reveal the location of specific nucleic acids sequences on chromosomes or in tissues, a crucial step for understanding the organization, regulation and function of genes. This popular, hands-on guide has been thoroughly updated to cover the key techniques currently in use. These include in situ hybridization to mRNA with oligonucleotide and RNA probes (both radio labelled and hapten labelled); analysis with light and electron microscopes; whole mountin situ hybridization; double detection of RNAs and RNA plus protein; and fluorescentin situ hybridization to detect chromosomal sequences. The protocols are complemented by advice on strategies for successful results, descriptions of the theoretical basis for the methods, and important new developments in gene expression databases. Collecting reliable and well-tested techniques, this volume will prove an essential tool for a wide range of biomedical researchers.

Publisher: Oxford ; New York : Oxford University Press, 1998
Edition: 2nd ed
ISBN: 9780585483788
0585483787
Characteristics: 1 online resource (xviii, 224 pages) : illustrations (some color)
Additional Contributors: Wilkinson, D. G.

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